Is There a Relationship between Nitrogen Intake and Testicular Urea Concentration?

نویسندگان

  • Ali Moharrery
  • Mohammad Javad Zamiri
چکیده

An experiment was conducted to determine the relationship between nitrogen intake and testicular urea content. Twenty ram lambs of fat-tailed Iranian native breed were used in this study. The mean (±SD) age and body weight of the lambs were 180±20 days and 36.7±6.3 kg, respectively. The lambs were divided in to five groups of equal size, and received a ration containing a single protein source; including: 1) urea as a control, 2) barley grain, 3) cotton seed meal, 4) gluten meal, and 5) canola meal. Crude protein content of the diet was adjusted at 13.6 percent, but with different ruminal degradability. During the experiment, the lambs were kept in isolation to avoid exposure to the sight and smell of female sheep. Rams were kept under natural day light conditions and ambient temperature (latitude 51◦27_N) for 11 weeks. On day 75, ruminal fluid samples were collected at 2, 4, 6, and 10 hours after morning feeding. On day 76, jugular blood samples were collected in vacuum tubes containing heparin as the anti-coagulant, and slaughtered subsequently. The right testis was removed, weighed, homogenized in phosphate buffer solution, and centrifuged to collect the supernatant for future analysis. The testicular extract and plasma were analyzed for urea nitrogen, and the ruminal fluid sample and testicular extract were analyzed for ammonia concentration. All samples were assayed in duplicate. The level of nitrogen intake significantly affected the testicular urea concentration (P<0.05). Plasma urea concentration was significantly correlated with rumen ammonia concentration at 4 and 6 hours, but not at 2 and 10 hours, after feeding. Testicular urea and ammonia concentrations were highly correlated. In conclusion, plasma urea concentration did not impact on testicular urea and ammonia concentrations but, nitrogen intake had a significant effect on testicular urea concentration.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Evaluation of Dietary Protein Intake by Calculation of Urea Generation Rate in Chronic Dialysis Patients in Kerman, 2002

Malnutrition is a common problem in chronic dialysis patients. There are several methods for nutritional assessment of hemodialysis patients, such as calculation of urea generation rate. As urea is produced from proteins catabolism and there is a linear correlation between urea generation and protein catabolic rate (PCR), PCR can be calculated by considering urea nitrogen appearence during the ...

متن کامل

Technical note: Nitrogen isotopic fractionation can be used to predict nitrogen-use efficiency in dairy cows fed temperate pasture.

The objective of this study was to investigate the relationship between nitrogen isotopic fractionation (δ(15)N) and nitrogen-use efficiency (milk nitrogen/nitrogen intake; NUE) in pasture-fed dairy cows supplemented with increasing levels of urea to mimic high rumen degradable protein pastures in spring. Fifteen cows were randomly assigned to freshly cut pasture and either supplemented with 0,...

متن کامل

Using blood urea nitrogen to predict nitrogen excretion and efficiency of nitrogen utilization in cattle, sheep, goats, horses, pigs, and rats.

The objectives of this study were to evaluate the potential for using blood urea N concentration to predict urinary N excretion rate, and to develop a mathematical model to estimate important variables of N utilization for several different species of farm animals and for rats. Treatment means (n = 251) from 41 research publications were used to develop mathematical relationships. There was a s...

متن کامل

Effect of Dietary Crude Protein Level on UT-B Expression and Nitrogen Efficiency in Growing Baluchi Male Lambs Fed Low or High Concentrate Diets

An experiment was carried out to evaluate how interactions between forage to concentrate ratio and dietary crude protein level may alter nitrogen efficiency and UT-B expression in growing Baluchi male lambs. Four Baluchi male lambs [30 ± 2 kg BW] were used in a 4 × 4 latin square design with 28-d periods and a 2 × 2 factorial arrangement of dietary treatments. The treatments fed forage: concent...

متن کامل

Effect of diet on creatinine clearance and excretion in young and elderly healthy subjects and in patients with renal disease.

Thirty-seven young healthy subjects with normal renal function were studied to assess the quantitative effect of protein intake on creatinine clearance. A standard 24-h urine collection and blood sample at the end of the collection were obtained for creatinine and urea concentrations. Correlations between creatinine clearance and urinary urea nitrogen excretion (r = 0.8; P less than 0.0001) and...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2017